Amyloid peptides (Abeta; Aβ) result from cleavage of amyloid precursor protein (APP) by beta and gamma secretase.The 42-amino acid variant, amyloid β peptide 1–42 is a widely accepted key biomarker for Alzheimer’s disease (AD) together with the total tau (T-tau) and phosphorylated tau (P-tau) protein. Diagnostic accuracy of the Aβ 1–42 /Aβ 1–40 ratio in cerebrospinal fluid (CSF)
compared to the concentration of Aβ 1–42 alone was found to be even more significant.
Currently these peptides are analyzed routinely in CSF by immunoassays, but in recent years, liquid chromatography tandem mass spectrometry (LC-MS/MS) has been investigated for the quantification of Aβ1-38, Aβ1-40, and Aβ1–42.
[Blennow 2010, Lewczuk 2015, Lame 2011]
Our neighbour CRO, Swiss BioQuant, use the Zirconium pump with a new scheme for loading and analyzing samples at the same time using the same binary pump. Dead times of the MS are greatly reduced, so they save a lot of instrument time while achieving exceptional sensitivity and precision.
Prolab Instruments has released a new software interface for its Zirconium Nano-UHPLC pump series for use in chromatography data systems from various vendors. This was requested by many users, and adds to the flexibility of LC system integration. Continue reading Zirconium Software Integrations for OpenLab, Analyst, Chromeleon, HyStar
The large sample sets produced by modern biological research often need to be analyzed in a minimal amount of time. Additionally, target compound concentrations in these samples may be very low. Continue reading Nano-HPLC: Why use smaller ID Columns and smaller Flow Rates in LC?