Category Archives: HPLC

HPLC Innovations at HUPO 2018

If you are coming to Orlando for HUPO 2018, you will be among the first to hear about our latest exciting developments to improve HPLC injections. You can find us at booth #305.

Also, we will have an oral presentation on the “innovation stage”, Tuesday October 2nd at 9:45 a.m.

If you are looking for applications of Prolab’s Zirconium pump for the lower nano-flow range in the field of proteomics, check out the posters and orals with Bruker’s nanoElute:

Poster 223: Short LC-gradients for high throughput and deep shotgun proteomics using PASEF on a TIMS equipped QTOF

Poster 251: High Sensitivity Phosphoproteomics using PASEF on a TIMS-QTOF mass spectrometer

Poster 296: Evaluating the timsTOFPRO bottom-up proteomics platform potential for Proteoform Profiling and Top-Down approaches

Poster 319: Highly reproducible and accurate label free quantification using the PASEF method on a TIMS-QTOF mass spectrometer

Oral WOC 11:58am, Wednesday – Oceana 6: PASEF for ultra-sensitive shotgun proteomics

Oral WOB pm 2:40pm, Wednesday – Oceana 7: Trapped ion mobility mass spectrometry for improved sensitivity and fastest proteomics

Photograph by Kelly DaaconLake Lucerne Reflections

Optimized SPE for Highly Organic Samples

A typical setup as seen in our previous application note employs three pumps – one for loading the sample out of the autosampler’s sample loop, one for diluting the sample’s organic solvent with water for trapping, and one for the analytical gradient, eluting the concentrated analytes off the trapping column onto an analytical column for separation.

We optimized this design a little, replacing the loading and dilution pumps by a single pump. See how it works in our new animation (turn on sound and/or subtitles):

Application Note: Quantification of Amyloid Beta Peptides in Human Cerebrospinal Fluid using Micro HPLC-HRMS

Amyloid peptides (Abeta; Aβ) result from cleavage of amyloid precursor protein (APP) by beta and gamma secretase.The 42-amino acid variant, amyloid β peptide 1–42 is a widely accepted key biomarker for Alzheimer’s disease (AD) together with the total tau (T-tau) and phosphorylated tau (P-tau) protein. Diagnostic accuracy of the Aβ 1–42 /Aβ 1–40 ratio in cerebrospinal fluid (CSF)
compared to the concentration of Aβ 1–42 alone was found to be even more significant.

Currently these peptides are analyzed routinely in CSF by immunoassays, but in recent years, liquid chromatography tandem mass spectrometry (LC-MS/MS) has been investigated for the quantification of Aβ1-38, Aβ1-40, and Aβ1–42.

[Blennow 2010, Lewczuk 2015, Lame 2011]

Amyloid-beta-42_1IYT
Figure 1: Solution structure of the Alzheimer’s disease amyloid beta-peptide (1-42)

Continue reading Application Note: Quantification of Amyloid Beta Peptides in Human Cerebrospinal Fluid using Micro HPLC-HRMS