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POSTER: Integrated Zirconium Low-Flow LC-MS Front-End

ASMS 2019 Prolab Corporate Poster

For everyone who did not attend the annual ASMS conference in Atlanta, we have put our poster about the new integrated low-flow UHPLC system online for download.

The poster highlights some of the many innovations implemented in this unique instrument: Minimized volumes save cycle time and contaminated surface. An optimized wash procedure for most effective and quick cleaning. An injection mechanism that protects hydrophilic analytes from the harms of pressure fluctuations. And waste liquid management that keeps your lab bench clean and tidy!

Optimized SPE for Highly Organic Samples

A typical setup as seen in our previous application note employs three pumps – one for loading the sample out of the autosampler’s sample loop, one for diluting the sample’s organic solvent with water for trapping, and one for the analytical gradient, eluting the concentrated analytes off the trapping column onto an analytical column for separation.

We optimized this design a little, replacing the loading and dilution pumps by a single pump. See how it works in our new animation (turn on sound and/or subtitles):

Application Note: Quantification of Amyloid Beta Peptides in Human Cerebrospinal Fluid using Micro HPLC-HRMS

Amyloid peptides (Abeta; Aβ) result from cleavage of amyloid precursor protein (APP) by beta and gamma secretase.The 42-amino acid variant, amyloid β peptide 1–42 is a widely accepted key biomarker for Alzheimer’s disease (AD) together with the total tau (T-tau) and phosphorylated tau (P-tau) protein. Diagnostic accuracy of the Aβ 1–42 /Aβ 1–40 ratio in cerebrospinal fluid (CSF)
compared to the concentration of Aβ 1–42 alone was found to be even more significant.

Currently these peptides are analyzed routinely in CSF by immunoassays, but in recent years, liquid chromatography tandem mass spectrometry (LC-MS/MS) has been investigated for the quantification of Aβ1-38, Aβ1-40, and Aβ1–42.

[Blennow 2010, Lewczuk 2015, Lame 2011]

Amyloid-beta-42_1IYT
Figure 1: Solution structure of the Alzheimer’s disease amyloid beta-peptide (1-42)

Continue reading Application Note: Quantification of Amyloid Beta Peptides in Human Cerebrospinal Fluid using Micro HPLC-HRMS