A typical setup as seen in our previous application note employs three pumps – one for loading the sample out of the autosampler’s sample loop, one for diluting the sample’s organic solvent with water for trapping, and one for the analytical gradient, eluting the concentrated analytes off the trapping column onto an analytical column for separation.

We optimized this design a little, replacing the loading and dilution pumps by a single pump. See how it works in our new animation (turn on sound and/or subtitles):